Determination of triptonide by cloud point extraction combined with MEKC.
A method has been developed for the determination of triptonide in the traditional Chinese herb Tripterygium wilfordii Hook F. by micellar electrokinetic capillary chromatography combined with cloud point extraction. The analyte was extracted at pH 3.0 by micelles of the nonionic surfactant polyoxyethylene […]
Biochemistry: H. Gröger awarded / Literature: Prize for K. Roth / ADUC Prizes.
Comparison of partial filling MEKC analyses of steroids with use of ESI-MS and UV spectrophotometry.
Until now, partial filling micellar EKC-ESI-MS (PF-MEKC-ESI-MS) has seldom been applied for human biomolecules. In this study, determination of three electrically neutral endogenous steroid hormones, namely androstenedione, testosterone, and epitestosterone, by PF-MEKC-ESI-MS was investigated. Since ESI-MS and ESI-MS/MS […]
Spotlights on our sister journals: Angew. Chem. Int. Ed. 14/2008.
Combinatorial Transition-Metal Catalysis: Mixing Monodentate Ligands to Control Enantio-, Diastereo-, and Regioselectivity.
This review focuses on a new approach to combinatorial homogeneous transition-metal catalysis which goes beyond the traditional parallel preparation of modular ligands. It is based on the use of mixtures of monodentate ligands L(a) and L(b), which upon exposure to a […]
Editorial: J. Sep. Sci. 5/2008.
Otsuka K.
Electrokinetic chromatography with polymeric pseudostationary phases.
Recent research and development efforts concerning polymeric pseudostationary phases (PSPs) for electrokinetic chromatography are reviewed. The introduction of new materials, characterization of structural effects on performance and selectivity, applications, and the use of polymeric PSPs with mass spectrometric detection are considered. Very interesting results concerning the effects […]
Utility of separation science in metabolomic studies.
Metabolomics, or alternately metabonomics, an emerging field of biochemical research, is a complementary technique to genomics, transcriptomics, and proteomics. Direct quantitative measurements of metabolite expressions in urine, serum, plasma, and tissue are essential for the study of biological processes in normal and disease states. Since the […]
Preview: Angew. Chem. Int. Ed. 15/2008.
Systematic characterization of the murine mitochondrial proteome using functionally validated cardiac mitochondria.
Mitochondria play essential roles in cardiac pathophysiology and the murine model has been extensively used to investigate cardiovascular diseases. In the present study, we characterized murine cardiac mitochondria using an LC/MS/MS approach. We extracted and purified cardiac mitochondria; validated their functionality […]
